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Objective@#To investigate the expression of SnoN in human hypertrophic scar fibroblasts and the mechanism of its participation in hypertrophic scar formation.@*Methods@#Eight patients with hypertrophic scar after burn in need of surgery were admitted in our unit from January to October 2013, and then hypertrophic scar tissue and normal skin tissue of full-thickness skin donor site resected by surgery of the patients were collected. Hypertrophic scar fibroblasts and normal skin fibroblasts of patients were isolated with method of explant culture and then sub-cultured. Cells of the third to fifth passage were used in the following experiments. (1) The protein expressions of SnoN of hypertrophic scar fibroblasts and normal skin fibroblasts were assessed with Western blotting. (2) The mRNA expressions of SnoN of another batch of hypertrophic scar fibroblasts and normal skin fibroblasts were determined with reverse transcription polymerase chain reaction. (3) Another batch of hypertrophic scar fibroblasts and normal skin fibroblasts were treated with 10 ng/mL transforming growth factor beta1 (TGF-β1) for 30 min, 1 h, 2 h, and 6 h, respectively, and then the protein expressions and mRNA expressions of SnoN of untreated cells and treated cells were detected as above. Data were processed with one way analysis of variance and independent sample t test.@*Results@#(1) The protein expression of SnoN of hypertrophic scar fibroblasts was 0.020±0.003, significantly lower than that of normal skin fibroblasts (0.032±0.005, t=7.19, P<0.05). (2) The mRNA expression of SnoN of hypertrophic scar fibroblasts was 0.407±0.157, with no significant difference from that of normal skin fibroblasts (0.339±0.095, t=-1.29, P>0.05). (3) The protein expression of SnoN of normal skin fibroblasts was increased in a time-dependent fashion with the TGF-β1 stimulation, and the protein expressions of SnoN of cells treated with TGF-β1 for 30 min, 1 h, 2 h, and 6 h were significantly higher than those of untreated cells (with t values from 2.27 to 27.89, P values below 0.05). The protein expression of SnoN of hypertrophic scar fibroblasts was decreased in a time-dependent fashion with the TGF-β1 stimulation, and the protein expressions of SnoN of cells treated with TGF-β1 for 30 min, 1 h, 2 h, and 6 h were obviously lower than those of untreated cells (with t values from 10.80 to 13.85, P values below 0.05). (4) The mRNA expressions of SnoN of normal skin fibroblasts and hypertrophic scar fibroblasts were both increased in a time-dependent fashion with the TGF-β1 stimulation, and the mRNA expressions of SnoN of the two types of cells treated with TGF-β1 for 30 min, 1 h, 2 h, and 6 h were both significantly higher than those of untreated cells (with t values from 18.16 to 58.22, P values below 0.05).@*Conclusions@#The protein expression of SnoN in hypertrophic scar fibroblasts is reduced, which weakens its inhibitory effect on TGF-β1 signal, thus amplifying the TGF-β1 signal, and it may participate in the formation of hypertrophic scar.
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Information resources of health education , disease knowledge and drug knowledge extracted by seconda-ry development fromhospital electronic librarywere disseminated on the Website , WIFI and WeChat platforms of People's Armed Police for the self-learning of users according to the demand of health dissemination and education in hospitals and health education at grass-roots level, in order to improve the efficiency and service of health edu-cation, solve the lack of resources and talents for health education, and to enhance the health service support ability for People's Armed Police.
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This study aimed to modify the mixed and purified culture of rat retinal ganglion cells (RGCs) in vitro. The retinae of 1-3 day old Sprague-Dawley (SD) rats were separated bluntly into two layers: inner layer and outer layer, under a surgical microscope. Retinal cells isolated from different layers (inner layer, outer layer and whole retinal tissue) by using enzyme dissociation method were cultured in F12/DMEM medium containing 15% FBS. After 3-day culture, the RGCs in the retinal cells obtained from mixed culture of inner, outer, and whole retinal tissue were identified by immunocytochemical staining of Thy-1.1, and the rate of RGCs to retinal cells (RGCs%) was calculated. Two monoclonal antibodies, anti-macrophages/granulocytes (OX-41) against rat macrophage and antibody against rat Thy-1.1 (OX-7), were used to purify RGCs by either a conventional or modified two-stepped immunopanning procedure (purification in situ). Purified RGCs were seeded at different cell density and cultured in F12/DMEM medium containing 15% FBS. Immunocytochemical staining for Thy-1.1, MTT, and PI-Hoechst33342 fluorescence imaging were used to identify the purity and the viability of RGCs in purified culture of RGCs. The results showed: (1) Immunocytochemistry of different retinal tissue layers culture revealed that the RGCs% was (19.9±1.2)%, (0.5±0.2)%, and (6.2±1.7)% respectively in the mixed culture of inner, outer, and whole retinal tissue, with differences being significant (P<0.05); (2) fluorescent double staining of Hoechst33342 and PI indicated that with the same RGCs%, RGCs obtained from purification in situ grew well with more neurite outgrowth than those by the conventional two-stepped immunopanning method; (3) the viability of purified RGCs seeded at high density was increased and the cells developed complex intercellular networks. The viability of RGCs was declined with the decreasing seeding density, and most cells presented round or oval in shape with thin neurites. It was concluded that: (1) RGCs% in the inner layer retina was higher than that in the outer layer retina; (2) RGCs obtained by in situ purification had more neurite outgrowth and lower mortality than those by conventional two-stepped immunopanning procedure; (3) the viability of purified RGCs could be increased by increasing cell seeding density to some extent.
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The intelligent level of the modern medical equipments is higher than before and the automatic calibration circuits are largely used. The control circuit of the intelligent equipments is quite complicated and its working modes have been simplified to four-terminal or double terminal nets. The intelligent medical equipments such as CT, MRI, DR, PET/ CT have more chances to have some "soft" errors that are usually related to the zero-line calibration. In actual use, it can be noticed and performed, and the detecting and servicing for the medical equipments can be much easier than before.
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To investigate the effect of proteasome inhibitor MG132 on the apoptosis of bovine lens epithelial cells (BLECs), the cells were treated with MG132 at different concentrations for12, 24 and 36 h. The cell viability was analyzed by MTT assay and the effect of MG132 on the apoptosis of BLECs was assessed by flow cytometry (FCM). The results showed that after treatment for the same period, the inhibitory effect of MG132 on BLECs proliferation was enhanced with the increment of the concentration of MG132 (0, 2, 5, 10, mumol/L) (P<0.05). The 50% inhibiting concentration (IC(50)) was 2.03 mumol/L when the BLECs were treated with MG132 for 36 h. MG132 also induced the apoptosis of BLECs obviously. FCM showed that the apoptosis index of the cells treated by MG132 at 2 mumol/L for 12 h was (20.24+/-1.51)%, and that of the control was (0.98+/-0.20)% respectively (P<0.01, n=3). It was concluded that MG132 could lead to apoptosis of BLECs. The decrease of proteasome activity may play an important role in the formation and development of cataract.
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As the base for the healthy development of civil medical equipment industry,the independent developing ability has attracted extensive attention.Firstly,medical equipment enterprises were divided into 5 modes according to their independent developing ability.Then the fuzzy evaluation method was utilized to evaluate them so as to correctly know the independent developing ability of medical equipment industry in our country.The evaluation shows that the brand value of the civil medical equipment is generally low.
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The quality of technicians is the dominate factor to determine the level of medical engineering section.The key to improving technical skills is to do basic jobs.During the Tenth Five-Year-Plan,we not only did many basic jobs and research works in science but did a lot of revolutions in order to accelerate the development of medical engineering and improve the working quality.Compared with the past,we have made great progress and development in standardized management,technology support,technician education,scientific researches,studies,technology level,the quality of service,etc.
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The paper puts forward the design and application of an imaging analysis system for micro-particles.The structure of the system,design theory and its application are discussed in the paper.
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Pressure vessel is used in medical organization as a kind of special medical equipment. It's easy to cause injury when improperly used. By analyzing technical performance, product security, accident threat that weakness element of MTF is pointed out and some suggestions of prevention accidents are produced.
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This paper introduces the method of electron tomography three-dimensional reconstruction,which is widely applied in structural biology field and summarizes its advantages and disadvantages.The aim is to provide a reference for the application of the method.
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The two important methods for displaying three-dimensional data called surface rendering and volume rendering are introduced and analyzed.It is concluded that volume rendering is superior to surface rendering for the former one provides a basis for visualization of three-dimensional data.
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The purpose of this paper is to develop a new dynamic image analyzing technique with the ability of real time measurement on red blood cell membrane minute fluctuation based on super resolution.The technique is proved strictly by correct theory,also its software realization as well as its advantage and disadvantage are also involved in.The results of applying the method in Aspergillus flavus cell(AFC)and red cell have proved its availability and achieved some new findings.
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The quality control methods are the important to improve the exactness of diagnses and the effectivity of treatment.X-ray imaging quality is an important factor in improving the X-ray diagnosis effect.To serve this purpose,medical engineering personnel should do the X-ray quality control regularly to keep every parameter in accordance with national standards and demands.The quality control methods for several important indexes of X-ray generators are also discussed.[Chinese Medical Equipment Journal,2008,29(2):99-101]
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Ambulance is a kind of new medical technique vehicle along with the development of medical science.Many countries are developing new ambulances in order to satisfy the demand of the emergency highway accident rescue.The GPS communication system and the vehicular medical power design are the most important parts of the ambulances for their complicated technology.This article discusses these two problems.